ASBC Method for microscopic yeast cell counting

An H-shaped moat form two counting areas, or plateaus. A “V” slash reduces the possibility of overflow.

• Cell Depth: 0.100mm +/- 2% (1/10mm)
• Volume: 0.1 Microliter
• Ruling Pattern: Improved Neubauer, 1/400 Square mm

Rulings cover 9 square millimeters. Boundary lines of the Neubauer ruling are the center lines of the groups of three. The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. The ruled surface is 0.10mm below the cover glass, so that the volume over each of the 16 small squares is .00025 cubic mm.

Cover slips for Hemacytometer (3048-13) to cover specimens in counting chambers are available. The cover glass is placed on the outer supports and due to their flatness, adheres very well. Thus, it forms an exact capillary gap defining the volume of the cell suspension over the counting grid. Made of high-quality optical glass. Ground and polished edges. Standard 22 X 22 mm size. Thickness 0.13 to 0.17 mm. 170 pieces of cover glass = 1oz.