Accuris High Fidelity DNA  Polymerase and Master Mix is designed for applications requiring highly accurate amplification and full control of enzyme concentration. This polymerase enzyme has been genetically modified for better solubility and higher activity across a broad range of ionic conditions, and will amplify a wide range of targets, including those that are GC or AT rich as well as crude DNA samples. A 3’-5’ proofreading exonuclease activity and an error rate of 4.55x10-7 makes the Accuris High Fidelity DNA Polymerase the perfect partner for cloning applications. The included 5X buffer contains an optimized concentration of dNTPs and enhancers for compatibility with a variety of applications up to 10kb in length.

The Accuris High Fidelity Master Mix is a 2X formulation providing extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, making it ideal for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors. The pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.

Accuris High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master-mix contains proprietary enhancers, and an antibody mediated hot-start polymerase with proof-reading component for trouble-free PCR reaction assembly and performance. The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.

Key Features

• Up to 100X higher fidelity than Taq polymerase
• Crude DNA samples will not inhibit amplification
• Ideal for cloning, mutagenesis, sequencing, and microarrays
• Options for blunt end or A-overhang products
• Optimized buffers include PCR enhancers
• Choose from Polymerase, Master Mix, or Hot Start Master Mix